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  • converting maq .map or mapview files to SINGLE-END mapview

    Hi all,

    I am trying to use a method in the ShortRead package in R, and for that, I need to input mapview files. I currently have paired-end mapview files, but the method does not support paired-end, and asks for single-end data.
    I understand that the paired-end data already has all the information for a single-end data, and was wondering how the formatting is different in the two.
    I could not find any mapview format for single-end data, and am willing to convert my paired-end mapview file to a single-end one. Could anyone help me by pointing me to an example of a single-end mapview file, or introducing me a method to do the above?

    the head of my paired-end mapview file looks like the following:

    SOLEXA10-24_4:6:988:1457/1 psr32 8 + 236 18 55 0 0 0 0 14 0 50 CTGaAgGCTGGGaAGaaGAAGggGaGcTGaaCTGGGaaGaTAaTaACTGg BA@:?9??@A@@;=A81@==<8;?3=7<<;;??@@?;7?8==:@8===B9 0

    Thanks a lot!

  • #2
    I just had to redo the mapping by forcing the aligner to use only single reads, not paired-end ones.
    As simple as that!

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