Hi all,
this may be something known... anyway, tags coming from Illumina sequencing method (GA II here) seem to map preferentially on forward strand... I haven't check if there is a statistically significant variation, but apparently tags aligned on forward strand are 2-5% more (in various ChIP-seq and also in genome resequencing).
I wonder which could be the source of this variation... The library prep? The cluster generation? The aligner? None of these steps makes sense to me...
d
this may be something known... anyway, tags coming from Illumina sequencing method (GA II here) seem to map preferentially on forward strand... I haven't check if there is a statistically significant variation, but apparently tags aligned on forward strand are 2-5% more (in various ChIP-seq and also in genome resequencing).
I wonder which could be the source of this variation... The library prep? The cluster generation? The aligner? None of these steps makes sense to me...
d
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