Hey guys,
Once we got differentially expressed (DE) genes using RNA-Seq, we need to do qPCR validation.
1.For a non-model plant, among the DE genes, maybe most of them are unknown. If you pick some known and unknown genes/transcripts to do qPCR validation, how do you design primers for those unknown (maybe novel) genes/transcripts? (qPCR primers span introns)
2.Generally, how many genes should be selected as candidate genes?
Any suggestions would be greatly appreciated.
Thanks.
Sincerely,
lzsph
Once we got differentially expressed (DE) genes using RNA-Seq, we need to do qPCR validation.
1.For a non-model plant, among the DE genes, maybe most of them are unknown. If you pick some known and unknown genes/transcripts to do qPCR validation, how do you design primers for those unknown (maybe novel) genes/transcripts? (qPCR primers span introns)
2.Generally, how many genes should be selected as candidate genes?
Any suggestions would be greatly appreciated.
Thanks.
Sincerely,
lzsph
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