Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Paired end solexa (goat/gerald) pipeline

    Hi,

    I am unable to go beyond the Solexa pipeline on paired end data. The data is 72 cycles of images in a single folder (36 x 2). I am not really sure of what the config.txt file should be!
    Any ideas?

    $ cat config.txt
    ANALYSIS sequence_pair
    USE_BASES Y35n,Y35n
    SEQUENCE_FORMAT --fastq
    ELAND_MULTIPLE_INSTANCES 6

    This one says:
    Found 1 reads.
    Read 1: length 72.
    Overriding lengths using option 72.
    Error: Too many reads specified in USE_BASES=Y35n,Y35n.
    Removing temporary file /tmp/tmp-GERALD-4440-4807.txt
    Error: ../Gerald/GERALD.pl (/data3/Solexa2/Vasatzis/111408_30J7KAAXX_300164_20/Data/config.txt) returned error code 2
    Error: Failed to process Gerald configuration.
    --
    bioinfosm

  • #2
    Try just:
    ANALYSIS sequence_pair
    SEQUENCE_FORMAT --fastq
    nothing else.

    Comment


    • #3
      There should be a file called PairedEndInfo.xml in the run folder that the pipeline looks for to know the sequences length. Is this file present and you are using GAPipeline 1.0?

      Comment


      • #4
        PairedEndInfo.xml it was!

        Although mine read like this-
        $ cat ../../PairedEndInfo.xml
        <?xml version="1.0"?>
        <FirstRead Length="36" />

        I added the line
        <SecondRead Length="36" />
        --
        bioinfosm

        Comment


        • #5
          ___________________________
          Last edited by bioinfosm; 02-02-2009, 12:50 PM. Reason: <silly me error>
          --
          bioinfosm

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM
          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          26 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          29 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          25 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          52 views
          0 likes
          Last Post seqadmin  
          Working...
          X