Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Index bias for TruSeq small RNA sample prep

    Hi Everyone,

    Although I have been visiting the site for quite some time, this is my first time posting on seqanswers. To get down to it, I have a specific question about using Truseq indexing primers for differential miRNA expression analysis on the Hiseq 2000 machine. As I understand it indexing primers can cause differences in amplification efficiency for different sequences within a library and these differences will distort the library in different ways depending on the indexing primer. So, the end result would be that one cannot accurately compare samples from different conditions that use different indexing primers (I hope that's not too convoluted). I could be wrong about this as perhaps illumina has done something to alleviate bias generated through amplification with differing indexing primers. I guess what I'm wondering: is this a legitimate concern? If so would swapping indexing primers between conditions from one run to the next alleviate this problem?

    Thanks in advance,
    Graduate Student

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
66 views
0 likes
Last Post seqadmin  
Working...
X