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Old 09-06-2011, 02:00 AM   #1
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Default PubMed: Barcoded primers used in multiplex amplicon pyrosequencing bias amplification

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Barcoded primers used in multiplex amplicon pyrosequencing bias amplification.

Appl Environ Microbiol. 2011 Sep 2;

Authors: Berry D, Ben Mahfoudh K, Wagner M, Loy A

Abstract
'Barcode-tagged' PCR primers used for multiplex amplicon sequencing generate a thus far overlooked amplification bias that produced variable T-RFLP and pyrosequencing data from the same environmental DNA template. We propose a simple 2-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyrosequencing libraries.


PMID: 21890669 [PubMed - as supplied by publisher]



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