Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Strand specific RNA-seq data from dUTP protocol ZhengXia Bioinformatics 8 10-16-2014 05:36 AM
RNA-Seq: High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation. Newsbot! Literature Watch 1 01-22-2013 12:48 AM
Strand-specific library appears not strand-specific oligo Illumina/Solexa 7 12-08-2011 10:54 AM
RNA-Seq: A Strand-Specific Library Preparation Protocol for RNA Sequencing. Newsbot! Literature Watch 0 09-29-2011 07:00 AM
Strand-preserving protocol for Illumina, 3'bias das RNA Sequencing 0 06-23-2009 10:39 AM

Thread Tools
Old 06-28-2010, 07:56 AM   #1
Location: USA

Join Date: Mar 2010
Posts: 55
Default strand-specific Illumina protocol

Is there any way to tell from the sequence data whether an RNA-Seq Illumina run was done on strand specific data or not?

PFS is offline   Reply With Quote
Old 06-28-2010, 08:53 AM   #2
Lee Sam
Location: Ann Arbor, MI

Join Date: Oct 2008
Posts: 57

I would assume that you could tell by looking at the reads aligning to the genes. If there's a mix of forward and reverse aligning reads, I might assume that the library was not prepped using a strand-specific protocol.
Lee Sam is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:48 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO