Hi All,
I am new to this forum. I have recently started using AB Solid V 2.0 for ChIP seq of a Drosophila TF. I used 500 bp avg Chromatin for pull down and for the library preparation it was brought down to ~100-200bp.
We ran MACS with default parameters on the data. I was surprised to see that mean peak length for Test IP vs.Input was ~1.6 Kb! (Similar results for Test IP Vs. Mock IP) There were peaks as big as 10 Kb at times. Is this common? How can I get better resolution? Please help!
I am new to this forum. I have recently started using AB Solid V 2.0 for ChIP seq of a Drosophila TF. I used 500 bp avg Chromatin for pull down and for the library preparation it was brought down to ~100-200bp.
We ran MACS with default parameters on the data. I was surprised to see that mean peak length for Test IP vs.Input was ~1.6 Kb! (Similar results for Test IP Vs. Mock IP) There were peaks as big as 10 Kb at times. Is this common? How can I get better resolution? Please help!
Comment