hi,
i wonder to know what should i do before mapping reads to reference genome?
reads is like:
>853_1434_503_F3
T00010230120200223112313302010303131
>853_1434_640_F3
T10110321132100321101032203211113333
>853_1434_723_F3
T10002311323331221330201030303112312
>853_1434_796_F3
T33330210031203013231322323302010303
>853_1434_1327_F3
T11231011321333211013021301000330201
these reads are for microRNA analysis, while as we known, microRNA's length is approximate 20nt, my reads are 35bp, should i shorten them?
i wonder to know what should i do before mapping reads to reference genome?
reads is like:
>853_1434_503_F3
T00010230120200223112313302010303131
>853_1434_640_F3
T10110321132100321101032203211113333
>853_1434_723_F3
T10002311323331221330201030303112312
>853_1434_796_F3
T33330210031203013231322323302010303
>853_1434_1327_F3
T11231011321333211013021301000330201
these reads are for microRNA analysis, while as we known, microRNA's length is approximate 20nt, my reads are 35bp, should i shorten them?
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