We plan to use uniq adaptor tagged primers for a PCR and then pool PCR products of about 5 samples together for one TruSeq index and follow the TruSeq protocol skipping fragmentation.
Pooling similar or near equal amounts of PCR products (purified) would be a key issue. I would be grateful for reccomendations on quantification methods. And the actual risk of using nano drop for quantitication. Would I be digging my own grave if I use nano drop ? Using any other method would be an additional expense associated
Pooling similar or near equal amounts of PCR products (purified) would be a key issue. I would be grateful for reccomendations on quantification methods. And the actual risk of using nano drop for quantitication. Would I be digging my own grave if I use nano drop ? Using any other method would be an additional expense associated
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