Hi Colleagues,
If I am not behind the times, none of the currently available sequencing platforms can manage the entire human genome as single sample in single lane.
At the same time, there is an option of running aliquots from the same library in several lanes. The data collected from individual lanes is merged for downstream analysis or comparison.
Running aliquots from the same library in the eight available lanes of one HiSeq or Genome Analyzer IIx flowcell will enhance the coverage of the entire human genome up to 30-fold (see the Illumina spec sheet for HiSeq 2000).
This coverage might be enough to bring forth the accurate and reproducible data on any significant part of the human genome.
Thus, the multilane approach might enable skipping the enrichment entirely or simplifying it.
I realize that it would add considerably to the price/running cost of the analysis. However, the enhanced price of taking up the several lanes for single library might be balanced by reduced occurrence of failed runs and, also, by taking a good deal of money and trouble off the enrichment.
I am interested in personal experience as well as in a reference to any publicly available information.
If I am not behind the times, none of the currently available sequencing platforms can manage the entire human genome as single sample in single lane.
At the same time, there is an option of running aliquots from the same library in several lanes. The data collected from individual lanes is merged for downstream analysis or comparison.
Running aliquots from the same library in the eight available lanes of one HiSeq or Genome Analyzer IIx flowcell will enhance the coverage of the entire human genome up to 30-fold (see the Illumina spec sheet for HiSeq 2000).
This coverage might be enough to bring forth the accurate and reproducible data on any significant part of the human genome.
Thus, the multilane approach might enable skipping the enrichment entirely or simplifying it.
I realize that it would add considerably to the price/running cost of the analysis. However, the enhanced price of taking up the several lanes for single library might be balanced by reduced occurrence of failed runs and, also, by taking a good deal of money and trouble off the enrichment.
I am interested in personal experience as well as in a reference to any publicly available information.
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