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Old 12-03-2017, 03:17 PM   #1
Meyana
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Default Recipe for NexteraXT respuspension buffer?

So, I will be running out of resuspension buffer (RSB) for my NexteraXT kit before everything else...

Does anybody know what is in it or can guide me to some info on it?
Is it even advisable to make your own?

Thanks!
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Old 12-03-2017, 10:58 PM   #2
nucacidhunter
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If you are not doing bead normalisation step, any common buffer such as TE, TE0.1 or EB will do it. For bead normalisation, EB will be better choice as its pH (8.0) is the same as RSB (RSB is proprietary).
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Old 12-04-2017, 05:17 PM   #3
Meyana
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I will not do bead normalisation - I think I'll find some TE buffer

The TE0.1, what is the 0.1 in this regard?
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Old 12-04-2017, 06:12 PM   #4
nucacidhunter
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It contains 0.1 mM EDTA in comparison to TE with 1 mM EDTA.
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Old 12-05-2017, 03:32 PM   #5
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Thanks for your help!
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illumina, nextera, nextera xt, resuspension, resuspension buffer

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