Hi everyone, I'm new to sequencing and would like to do some full-length mRNA-seq. I was wondering if anyone could help me out with some pretty basic questions?
1. Do most people use Illumina's mRNA-seq kit, or do they buy the components themselves?
2. Is there much cost saving from not using the kit?
3. Does anyone have a complete list of buffer recipes, primer concentrations, etc? (I can't find that info from Illumina)
4. Are there any "tweaks" in Illumina's protocol that people have found useful?
Thanks!!
1. Do most people use Illumina's mRNA-seq kit, or do they buy the components themselves?
2. Is there much cost saving from not using the kit?
3. Does anyone have a complete list of buffer recipes, primer concentrations, etc? (I can't find that info from Illumina)
4. Are there any "tweaks" in Illumina's protocol that people have found useful?
Thanks!!