Hi all,
I am doing RNA-seq in shRNA treated cells.
Can I expect in RNA-seq data there is less shRNA target gene RNA abundance than the shRNA control?
However I read a few articles saying that shRNA is interfering translation not transcription. If so , I should see the similar shRNA targeted gene expression in specific shRNA sample and shRNA control sample.
Anyone knows this?
Thanks!
I am doing RNA-seq in shRNA treated cells.
Can I expect in RNA-seq data there is less shRNA target gene RNA abundance than the shRNA control?
However I read a few articles saying that shRNA is interfering translation not transcription. If so , I should see the similar shRNA targeted gene expression in specific shRNA sample and shRNA control sample.
Anyone knows this?
Thanks!