Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Megablast x dc-megablast

    When I should use discontinuous Megablast instead of Megablast? I do not know which blast is better to use for nucleotide blast.

    I read some information about it as follow:

    Megablast: Retrieves highly similar sequences and is very fast. It efficiently find long alignments between very similar sequences -- it is intended for comparing a query to closely related sequences and works best if the target percent identity is 95% or more. The MegaBLAST program is useful for extremely rapid scans of a nucleotide database with a large batch of nucleotide queries, such as EST sequences.

    Discontiguous megablast: for more dissimilar sequences, should be used for cross-species comparisons, allows for some mismatches. Retrieves more dissimilar sequences than megablast, but is more sensitive than blastn. It uses an initial seed that ignores some bases (allowing mismatches) and is intended for cross-species comparisons.

    So the main difference is - megablast is better for same species search and dc-megablast is better to search to other species? Anything else?

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X