Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • sra to Fastq files

    Hello,

    I don't understand how to use fastq-dump. All I get is errors.

    Code:
    $ ./fastq-dump -A SRR638709 SRR638709.sra 
    2013-08-16T20:23:37 fastq-dump.2.3.2 err: name not found while resolving tree within virtual file system module - failed to open 'SRR638709.sra'
    What am I doing wrong?

    Thanks

  • #2
    Have you tried

    Code:
    $ ./fastq-dump  /full_path_to/SRR638709.sra
    Several people have posted that providing full path to the SRA file seems to work due to some quirk in fastq-dump code.

    Comment


    • #3
      Originally posted by GenoMax View Post
      Have you tried

      Code:
      $ ./fastq-dump  /full_path_to/SRR638709.sra
      Several people have posted that providing full path to the SRA file seems to work due to some quirk in fastq-dump code.
      Partially worked. One thing (that wasn't obvious) was that one must copy the SRA file to the working directory (the BIN directory) and then run the *.jar file. LEAVE the window open.

      Now, one can run the fastq-dump script and it should work.

      Then it worked for me (Ubuntu 12.04.2 LTS AMD64).

      - ch

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      59 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      57 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      48 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X