Originally posted by quinlana
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How can I get a read count per exon per transcript in a particular gene from BAM file? I tried using htseq-count with id_attr=transcipt_id but this approach does not give me the read count per exon per transcript. I tried using DEXSeq to get the read count, but it does not get what I want. Is there any way to get the read count? I only got one gene of interest to analyse which TPM1. This gene has more than 40 isoforms, so it is possible to get each of the isoforms their read count for each exon. Thanks!
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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