I am at a point in my project where it would be exceedingly useful to perform RNA-seq on full human transcripts on the PacBio platform. Can anyone point me to a reference where someone has done this? I am considering various protocols to ensure cDNA synthesis goes to completion but would love to hear about how others are accomplishing this.
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Some useful references:
http://blog.pacificbiosciences.com/2...ll-length.html
Protocol using Clonetech kit:
http://www.smrtcommunity.com/Share/P...eName=Protocol
Analysis primer:
https://github.com/PacificBioscience...scriptome-data
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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