What pipette is recommended (or have you seen used) to pipette custom primer into the Illumina sequencing primer wells of a MiSeq? (See here :
if it isn't clear what I mean.)
Currently we use some 1ml glass serological pipettes that were purchased over a decade ago. They actually work, but only poorly. We pipet out the liquid in Cartridge Positions 12 and/or 13 and/or 14 into eppendorf tubes. Mix in the (concentrated) custom primers to 0.5uM and pipet the mixture back into the position from which it came.
But if there existed a long pipette tip that fit on the end of a normal pipetter (p1000), that would be preferable.
--
Phillip
if it isn't clear what I mean.)
Currently we use some 1ml glass serological pipettes that were purchased over a decade ago. They actually work, but only poorly. We pipet out the liquid in Cartridge Positions 12 and/or 13 and/or 14 into eppendorf tubes. Mix in the (concentrated) custom primers to 0.5uM and pipet the mixture back into the position from which it came.
But if there existed a long pipette tip that fit on the end of a normal pipetter (p1000), that would be preferable.
--
Phillip
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