SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: A new strategy for genome assembly using short sequence reads and reduced rep Newsbot! Literature Watch 1 11-18-2010 01:52 AM
PubMed: De novo assembly of short sequence reads. Newsbot! Literature Watch 0 08-21-2010 03:01 AM
PubMed: De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sord Newsbot! Literature Watch 0 04-14-2010 03:01 AM
De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads Michael.James.Clark Literature Watch 1 04-09-2010 01:16 PM
De novo assembly of human genomes with massively parallel short read sequencing dan Literature Watch 0 12-21-2009 05:40 AM

Reply
 
Thread Tools
Old 01-31-2010, 08:24 PM   #1
krobison
Senior Member
 
Location: Boston area

Join Date: Nov 2007
Posts: 747
Default Parallel, tag-directed assembly of locally derived short sequence reads.

Nat Methods. 2010 Feb;7(2):119-22. Epub 2010 Jan 17.
Parallel, tag-directed assembly of locally derived short sequence reads.
Hiatt JB, Patwardhan RP, Turner EH, Lee C, Shendure J.

[1] Department of Genome Sciences, University of Washington, Seattle, Washington, USA. [2] These authors contributed equally to this work.

We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.

PMID: 20081835

Last edited by krobison; 01-31-2010 at 08:26 PM. Reason: stupid typo (lost initial P) in title
krobison is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:13 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO