Hey guys,
I have RNASeq data with paired end read fastq files for case and control samples. I am a beginner and have never ran programs like tophat, cufflinks etc before.
I wanted to know how I can use Tophat to accurately map these read files to the reference genome hg 19 build(assume I have the two fastq files for each end post quality filtering). What else do I have to keep in mind if I want to perform a DE analysis in the following steps?
I have RNASeq data with paired end read fastq files for case and control samples. I am a beginner and have never ran programs like tophat, cufflinks etc before.
I wanted to know how I can use Tophat to accurately map these read files to the reference genome hg 19 build(assume I have the two fastq files for each end post quality filtering). What else do I have to keep in mind if I want to perform a DE analysis in the following steps?
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