Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Which PolyA kit to choose?

    Hi,
    For mRNA-Seq, I am looking for a poly(A) selection kit based on oligo(dT) magnetic beads.
    Any recommendations?
    Cheers,
    /Jakob

  • #2
    I have used this with good results:

    The Dynabeads mRNA DIRECT Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.

    Comment


    • #3
      The NEXTflex Poly(A) Beads from Bioo Scientific work quite well and are very cost-effective. As a disclaimer, I work for Bioo Scientific.

      Comment


      • #4
        Thanks for your input Ola and kerplunk412.
        I have ordered the Dynabeads - but will consider NEXTflex beads if the Dynabeads fails:-)
        Cheers, /Jakob

        Comment


        • #5
          We used KAPA one. It seems to be cost effective.

          Comment


          • #6
            Originally posted by kerplunk412 View Post
            The NEXTflex Poly(A) Beads from Bioo Scientific work quite well and are very cost-effective. As a disclaimer, I work for Bioo Scientific.
            How much? Can you provide a price?

            Comment

            Latest Articles

            Collapse

            • seqadmin
              Current Approaches to Protein Sequencing
              by seqadmin


              Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
              04-04-2024, 04:25 PM
            • seqadmin
              Strategies for Sequencing Challenging Samples
              by seqadmin


              Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
              03-22-2024, 06:39 AM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by seqadmin, 04-11-2024, 12:08 PM
            0 responses
            18 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-10-2024, 10:19 PM
            0 responses
            22 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-10-2024, 09:21 AM
            0 responses
            16 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-04-2024, 09:00 AM
            0 responses
            47 views
            0 likes
            Last Post seqadmin  
            Working...
            X