Hi everyone. I have some thoughts to use leftovers after several MiSeq runs to perform one extra run (I do have an extra flowcell, but reagents are needed). So my question is how much reagent is used by MiSeq per each cycle? Does anyone now that or have an experience in using the rest of the reagent after a run?
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How much MiSeq sequencing reagent is being used per each cycle.
Last edited by Mizraelson; 09-29-2017, 02:39 AM.Tags: None
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I understand being frugal is a good thing but trying to re-use reagents that are several days old and stayed out at 6-8 degrees in the machine does not seem like a wise choice unless you want to do this purely as an experiment (you will need to pool reagents from multiple runs unless you have a large amount left over due to a short run with a long run kit).
Generally all kits have enough extra reagents to do 10-15 cycles (to account for index reads) as I recall.
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