Hi everyone,
I want to use Miseq for PCR amplicon library. I would like to ask if it is possible to sequence a paired-end read with barcodes like the following structure:
[P5 tail][Seq primer 1][NNNN][Target primer Forward][Target][Target primer Reverse][NNNN][Seq primer 2][P7 tail]
Regards
seq-learner
I want to use Miseq for PCR amplicon library. I would like to ask if it is possible to sequence a paired-end read with barcodes like the following structure:
[P5 tail][Seq primer 1][NNNN][Target primer Forward][Target][Target primer Reverse][NNNN][Seq primer 2][P7 tail]
Regards
seq-learner
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