G'day Everyone,
I am new to genomics and hope that I don't annoy you by asking a basic question.
I am thinking of using RAD-seq SNP discovery to identify SNP markers for a population genetics project in a non-model species. At the moment, I am trying to figure out if it is realistic for me to go down this path and have been reading the protocol by Etter et al 2011. I have a question about pooling populations that I hope someone can help me with:
Can I pool the DNA of individuals before I digest the DNA with the appropriate restriction enzyme? Meaning I would only need to do 1 digest per library rather than ~20.
If not, what is the reason behind not doing this?
Thank you in advance for your help and time.
Kind regards,
Adam
I am new to genomics and hope that I don't annoy you by asking a basic question.
I am thinking of using RAD-seq SNP discovery to identify SNP markers for a population genetics project in a non-model species. At the moment, I am trying to figure out if it is realistic for me to go down this path and have been reading the protocol by Etter et al 2011. I have a question about pooling populations that I hope someone can help me with:
Can I pool the DNA of individuals before I digest the DNA with the appropriate restriction enzyme? Meaning I would only need to do 1 digest per library rather than ~20.
If not, what is the reason behind not doing this?
Thank you in advance for your help and time.
Kind regards,
Adam
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