mira assembly error

[szimmerman]

Hi everyone,

So I was doing a hybrid assembly with mira using a pacbio assembly as the backbone and Illumina reads in a .fasta file. Along with that fasta file, I have a .qual file, but it does not seem to be working. When I try running mira I get an error saying "Illegal character (@: 40) at begin of fasta integer value sequence in file at byte position 1." My current theory is that the quality file is using phred quality scoring instead of solexa which is needed. Is that correct? And if so, how can I convert the file to one that mira can read? The old version had this command ssiqf, but they seem to have taken it out in version 3.9.

Thanks!

[JackieBadger]

MIRAtalk mailing list will give you a quick response to fix your problem from the program authors

[szimmerman]

Thanks a lot!