This is my understanding for fragment and read. Is my understanding correct?
If a fragment is 1000 bp and the read is 150 bp, only the first 150 bp of the fragment is sequenced.
How is the rest 850 sequenced?
My understanding is the initial DNA have many copies and different copies are fragmented at different positions. So, let's say in to a whole genome, the 1st copy is fragmented into 1st bp - the 1000th bp, the 1001st - 2000th bp, et al, while the 2nd copy is fragmented into 59th bp - 1058th bp, the 1059th - 2058th bp et. al, and the 3rd copy is fragmented into 74th bp - 1073rd bp, 1074th bp - 2073rd bp et.al, the forth, the fifth ......
So, it can result in read overlapping and the reads can be assembled based on the overlapping.
Is my understanding correct?
If a fragment is 1000 bp and the read is 150 bp, only the first 150 bp of the fragment is sequenced.
How is the rest 850 sequenced?
My understanding is the initial DNA have many copies and different copies are fragmented at different positions. So, let's say in to a whole genome, the 1st copy is fragmented into 1st bp - the 1000th bp, the 1001st - 2000th bp, et al, while the 2nd copy is fragmented into 59th bp - 1058th bp, the 1059th - 2058th bp et. al, and the 3rd copy is fragmented into 74th bp - 1073rd bp, 1074th bp - 2073rd bp et.al, the forth, the fifth ......
So, it can result in read overlapping and the reads can be assembled based on the overlapping.
Is my understanding correct?
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