Hi everyone,
I have been having trouble with getting the protocol to work although it used to in the past. After a little bit of debugging/discussion, I believe that it might be due to bad adapters. So here's my question:
What is the shelf-life of Illumina adapters (in form of ordered oligos and then annealed) with repeated freezing and thawing?
Also is thawing in ice (4C) critical to keep them annealed or RT is fine as well?
Thanks for the advice!
I have been having trouble with getting the protocol to work although it used to in the past. After a little bit of debugging/discussion, I believe that it might be due to bad adapters. So here's my question:
What is the shelf-life of Illumina adapters (in form of ordered oligos and then annealed) with repeated freezing and thawing?
Also is thawing in ice (4C) critical to keep them annealed or RT is fine as well?
Thanks for the advice!
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