Hi
Has anyone attempted DNA extraction on small samples (say 500-1000 cells) for any NGS protocol?
We currently attempting to extract DNA from single human colonic crypts and for our targetted sanger sequencing its fine to use picopure but is it ok, or are there better methods? Most column based kits yield no to little DNA.
Any advice would be welcomed
Has anyone attempted DNA extraction on small samples (say 500-1000 cells) for any NGS protocol?
We currently attempting to extract DNA from single human colonic crypts and for our targetted sanger sequencing its fine to use picopure but is it ok, or are there better methods? Most column based kits yield no to little DNA.
Any advice would be welcomed
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