Hi all,
I am planning to run a small RNA Seq (TruSeq Small RNA Library Prep) in a Illumina MiSeq and reading the protocol they use a 5 um filter tube to transfer the elute and the gel debris following a centrifugation. Illumina recommends IST Engineering 5388-50.
I was wondering whether anyone has used another filter size and how it worked. I read that user huguesparri used 0.45 um Corning® Costar® Spin-X® centrifuge tube filters and it worked fine (http://seqanswers.com/forums/showthread.php?t=33987) but I would like to have more opinions. Any other alternatives would also be appreciated.
Thanks in advance
I am planning to run a small RNA Seq (TruSeq Small RNA Library Prep) in a Illumina MiSeq and reading the protocol they use a 5 um filter tube to transfer the elute and the gel debris following a centrifugation. Illumina recommends IST Engineering 5388-50.
I was wondering whether anyone has used another filter size and how it worked. I read that user huguesparri used 0.45 um Corning® Costar® Spin-X® centrifuge tube filters and it worked fine (http://seqanswers.com/forums/showthread.php?t=33987) but I would like to have more opinions. Any other alternatives would also be appreciated.
Thanks in advance
Comment