Hi-
Illumina recommends using between 10-50 ng of ChIP DNA for a sample prep. Does anyone have experience with using less than 10 ng? I tried using about 2 ng (it's all that I had), figuring the worst that could happen would be to get fewer reads than usual, but I ended up getting basically no reads at all. I calculated the amount of PCR-enriched DNA to load onto the flow cell based on the A280 reading. I couldn't see the PCR-enriched sample on the Bioanalyzer, but I figured that this wasn't a big deal, as another sample that gave excellent sequencing results was only barely detectable on the Bioanalyzer.
Does anyone know if there is a general problem with using less than 10 ng starting material?
Thanks!
Illumina recommends using between 10-50 ng of ChIP DNA for a sample prep. Does anyone have experience with using less than 10 ng? I tried using about 2 ng (it's all that I had), figuring the worst that could happen would be to get fewer reads than usual, but I ended up getting basically no reads at all. I calculated the amount of PCR-enriched DNA to load onto the flow cell based on the A280 reading. I couldn't see the PCR-enriched sample on the Bioanalyzer, but I figured that this wasn't a big deal, as another sample that gave excellent sequencing results was only barely detectable on the Bioanalyzer.
Does anyone know if there is a general problem with using less than 10 ng starting material?
Thanks!
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