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I have a batch of recent HiSeq paired end runs where the sequencing centre reported pre-phasing problems on one or both reads of some runs due to reagent issues. Should I expect to see this reflected in poorer quality scores in the FASTQ earlier in the reads? Basically I am trying to figure out how to recover usable data from the problem runs. I think that trimming + a quality filter should be ok if the pre-phasing is reflected in the quality scores the way I expect, but I am not familiar enough with the technology to be confident. Can anyone confirm if this is the right way to go?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM