Hello, does anyone know the cutoff of trimming length. I have Illumina data from 2X150bp and 2X250bp sequencing. I'd like to trim them. I set the Q score to 30. How about length? What is the cutoff do you normally use?
For example, for 2X150bp data, should I discard all the reads short than 75bp or 100bp? How about 2X250 bp? I just want know the cutoff people usually use? Phred score >30 is normally use. I am not sure about the length.
Thanks
For example, for 2X150bp data, should I discard all the reads short than 75bp or 100bp? How about 2X250 bp? I just want know the cutoff people usually use? Phred score >30 is normally use. I am not sure about the length.
Thanks
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