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  • liftOver: rtracklayer vs. UCSC binary

    Hi,

    I am just trying to liftover the following genomic region from hg18 to hg19.

    chr6 29677984 33485635

    When I used liftOver() in rtracklayer, I obtained the following results.

    > hla.gr.hg18 <- GRanges(seqnames="chr6",IRanges(29677984,33485635))
    > chain <- import.chain("./hg18ToHg19.over.chain")
    > hla.gr.hg19 <- liftOver(hla.gr.hg18,chain)
    > hla.gr.hg19
    GRangesList of length 1:
    $1
    GRanges with 8 ranges and 0 metadata columns:
    seqnames ranges strand
    <Rle> <IRanges> <Rle>
    [1] chr6 [29570005, 31963859] *
    [2] chr6 [31963877, 31964315] *
    [3] chr6 [31964332, 31964390] *
    [4] chr6 [31964396, 31996597] *
    [5] chr6 [31996615, 31997053] *
    [6] chr6 [31997070, 31997128] *
    [7] chr6 [31997133, 32012535] *
    [8] chr6 [32012537, 33377657] *

    When I used the binary for liftover from UCSC as follows,

    ./liftOver input.bed hg18ToHg19.over.chain output.bed unlifted.bed

    output.bed contained only one interval: chr6 29570005 33377657

    Can anyone explain to me the reason for this difference in liftover?

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