Hi All,
I've noticed that using the TruSeq genomic library prep kit, rather than my own stock of enzymes/materials, has yielded a much higher cluster density even when both samples are equimolar.
My theory is that the Illumina kit is working better than my own reagents, so the percent of successfully adapter-ligated DNA is higher, or somehow their proprietary blend of adapters work better than the ones I order from IDT. I do not have qPCR to quantify this, but I was just wondering if others had theories or noticed the same type of thing happening with their libraries.
Thanks!
I've noticed that using the TruSeq genomic library prep kit, rather than my own stock of enzymes/materials, has yielded a much higher cluster density even when both samples are equimolar.
My theory is that the Illumina kit is working better than my own reagents, so the percent of successfully adapter-ligated DNA is higher, or somehow their proprietary blend of adapters work better than the ones I order from IDT. I do not have qPCR to quantify this, but I was just wondering if others had theories or noticed the same type of thing happening with their libraries.
Thanks!
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