Hello Everyone,
I am a PhD student in Wildlife Biology at Texas A&M University-Kingsville. As part of my dissertation, I am planning to examine MHC diversity in some highly endangered and fragmented populations of ocelots.
At this point, I am trying to decide which sequencing method to use on my samples. We have an ABI 3130xL analyzer in the lab, which is probably sufficient for my purposes, but I'm not really looking forward to all that cloning.
I'm considering sending my samples in to a lab in order to have them sequenced on an IonTorrent PGM. Basically, it's going to come down to cost. I have a really small gene (238 bp) that I'm looking at, which can have up to 3 repeats per chromosome. If I can put unique barcode adapters on all my samples and multiplex them together that would be a lot easier than doing things the old school way. If anyone has any thoughts about this I'd love to hear them. This is a new field for me.
I am a PhD student in Wildlife Biology at Texas A&M University-Kingsville. As part of my dissertation, I am planning to examine MHC diversity in some highly endangered and fragmented populations of ocelots.
At this point, I am trying to decide which sequencing method to use on my samples. We have an ABI 3130xL analyzer in the lab, which is probably sufficient for my purposes, but I'm not really looking forward to all that cloning.
I'm considering sending my samples in to a lab in order to have them sequenced on an IonTorrent PGM. Basically, it's going to come down to cost. I have a really small gene (238 bp) that I'm looking at, which can have up to 3 repeats per chromosome. If I can put unique barcode adapters on all my samples and multiplex them together that would be a lot easier than doing things the old school way. If anyone has any thoughts about this I'd love to hear them. This is a new field for me.
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