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Hi everyone,
I have a question about RNA-seq.
"Some manipulations during library construction also complicate the analysis
of RNA-Seq results. For example, many shorts reads that are identical to each
other can be obtained from cDNA libraries that have been amplified. These could be a genuine reflection of abundant RNA species, or they could be PCR artefacts. One way to discriminate between these possibilities is to determine whether the same sequences are observed in different biological replicates.(Nat Rev Genet. 2009 Jan;10(1):57-63)"
Is there any other way in the above situation to discriminate between abundant RNA or PCR artefacts?
Thanks,
Hai-Ri Li
I have a question about RNA-seq.
"Some manipulations during library construction also complicate the analysis
of RNA-Seq results. For example, many shorts reads that are identical to each
other can be obtained from cDNA libraries that have been amplified. These could be a genuine reflection of abundant RNA species, or they could be PCR artefacts. One way to discriminate between these possibilities is to determine whether the same sequences are observed in different biological replicates.(Nat Rev Genet. 2009 Jan;10(1):57-63)"
Is there any other way in the above situation to discriminate between abundant RNA or PCR artefacts?
Thanks,
Hai-Ri Li