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Old 02-01-2015, 11:22 PM   #1
Yehudit
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Location: Israel

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Default minimum concentration or minimum number of DNA copies required by next gen sequencin

Hello,

1.What is the minimum concentration or minimum number of DNA copies required by next gen sequencing or standard sequencing machine (assuming I would like to sequence 10,000 bases or more)?

2. If I end up with clean ssDNA fragments, lengthen between 10,000-1,000,000bp (after column purification), what would be the best sequencing platform for me to use?

Thanks

Yeudit
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Old 02-10-2015, 07:33 AM   #2
Zaag
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Rule of thumb is if you can measure it you can sequence it.
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