Hello,
I have a question which I can guess the answer is no but I still ask...
Suppose I add one nucleotide to the 3' adaptor of the genomic DNA adaptor and the outcome will be:
5' AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCT (N) AGATCGGAAGAGCTCGTATG CCGTCTTCTGCTTGC 3'
instead of
5' AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCT (N) AGATCGGAAGAGCTCGTATG CCGTCTTCTGCTTG 3'
Should it interfere with the process?
Thanks for your help!
Shachar
I have a question which I can guess the answer is no but I still ask...
Suppose I add one nucleotide to the 3' adaptor of the genomic DNA adaptor and the outcome will be:
5' AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCT (N) AGATCGGAAGAGCTCGTATG CCGTCTTCTGCTTGC 3'
instead of
5' AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCT (N) AGATCGGAAGAGCTCGTATG CCGTCTTCTGCTTG 3'
Should it interfere with the process?
Thanks for your help!
Shachar