Hi,
I submitted a final contig from an uncultivable bacterial chromosome to BASys (since it is compulsory to check the gram staining box, i checked "positive" but in fact it cant be cultivated, so we dont know whether it s a gram negative or positive bacteria),
so,
the result came out and ALL annotation were for forward strand, no reverse strand.
could someone explain me, why?
I submitted a final contig from an uncultivable bacterial chromosome to BASys (since it is compulsory to check the gram staining box, i checked "positive" but in fact it cant be cultivated, so we dont know whether it s a gram negative or positive bacteria),
so,
the result came out and ALL annotation were for forward strand, no reverse strand.
could someone explain me, why?