Quantifying gene expression at the level of translation: Ribo-seq in tissue

DerSeb · 07-22-2015, 08:44 PM

We performed ribosome profiling of the heart and liver of two inbred rat strains. Our results suggest that:

- Ribo-seq is a better proxy for protein levels than RNA-seq
- There is widespread regulation of gene expression AFTER transcription
- Integration of RNA-seq and Ribo-seq reveals translational control and opens up a new level of gene regulation for further studies

Read the full manuscript here:

Translational regulation shapes the molecular landscape of complex disease phenotypes - Nature Communications

The Ribo-seq data used in this study is of course publicly available and we believe we have generated some of the deepest datasets to date with 5 biological replicates for each condition.

jack1090 · 09-25-2015, 11:30 PM

thanks DerSeb

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07-22-2015, 08:44 PM	#1
DerSeb Member Location: Singapore Join Date: Oct 2009 Posts: 44	Quantifying gene expression at the level of translation: Ribo-seq in tissue We performed ribosome profiling of the heart and liver of two inbred rat strains. Our results suggest that: - Ribo-seq is a better proxy for protein levels than RNA-seq - There is widespread regulation of gene expression AFTER transcription - Integration of RNA-seq and Ribo-seq reveals translational control and opens up a new level of gene regulation for further studies Read the full manuscript here: Translational regulation shapes the molecular landscape of complex disease phenotypes - Nature Communications The Ribo-seq data used in this study is of course publicly available and we believe we have generated some of the deepest datasets to date with 5 biological replicates for each condition.

09-25-2015, 11:30 PM	#2
jack1090 Junior Member Location: newyork Join Date: Sep 2015 Posts: 2	thanks DerSeb -------------------------------------------------------------------------------------- "Learn to be happy with what you have while you pursue all that you dream." University Physics with Modern Physics 14th edition pdf - Everyday Economics Honest Answers to Tough Questions Last edited by jack1090; 02-26-2016 at 03:36 AM.