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Old 10-02-2013, 07:21 AM   #1
A_Harrington
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Location: Saint Louis, MO

Join Date: Oct 2013
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Question Electropherogram of insect (S2) RNA

Hey Everyone,

I'm trying to make libraries from S2 cells and have a question regarding what the bioanalyzer electropherogram should look like. I know the RIN numbers for insect RNA are usually pretty low due to the dissociation of the 28S rRNA, but I can't seem to find any examples of "good" insect RNA for a visual comparison. I'm using the pico chip, RIN numbers are between 6-7.5, samples are not heat denatured prior to loading. Thanks!
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Old 10-07-2013, 12:59 AM   #2
ZFHans
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Location: Leiden

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Hi,

We once made some RNAseq libraries from Tribolium castaneum. A trace is added as attachment. Our Bioanalyzer got confused by the trace and refused to give a RIN value. We continued with library prep and got good libraries.
Hope this helps,

Hans Jansen
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