Hi,
I am attempting to feed my 454 sequencing data sets into MIRA for assembly.
Here is my data, all in .sff files:
Flx unpaired reads (6 separate. sff files)
Titanium unpaired reads (3 separate .sff files)
Titanium paired-end reads (2 separate .sff files).
I can extract to fasta and xml with the individual sets (unpaired-only and paired-only), but the MIRA assembler requires all data to be combined into a single data file.
Does anyone know if this can be done with sff_extract? If so, can you explain to me how? The sff_extract webpage doesn't explain how to do this.
I am attempting to feed my 454 sequencing data sets into MIRA for assembly.
Here is my data, all in .sff files:
Flx unpaired reads (6 separate. sff files)
Titanium unpaired reads (3 separate .sff files)
Titanium paired-end reads (2 separate .sff files).
I can extract to fasta and xml with the individual sets (unpaired-only and paired-only), but the MIRA assembler requires all data to be combined into a single data file.
Does anyone know if this can be done with sff_extract? If so, can you explain to me how? The sff_extract webpage doesn't explain how to do this.
Comment