Hi friends,
I prepared mulitplexing libraries for ChIP-Seq of a histone marker in different cell types. I used sonication as the chromatin shearing method and used the Truseq adaptors for generating the libraries. However, the size distribution of the libraries I made varied from one to the other with a maximum difference of about 40bp (bioanalyzer image attached). Could anyone here give me some suggestion as whether this would affect the outcome of the experiment? What would be a decent size difference to expect when doing multiplexing? I also found that the input samples tend to have more such variations.
Many thanks.
Ping
I prepared mulitplexing libraries for ChIP-Seq of a histone marker in different cell types. I used sonication as the chromatin shearing method and used the Truseq adaptors for generating the libraries. However, the size distribution of the libraries I made varied from one to the other with a maximum difference of about 40bp (bioanalyzer image attached). Could anyone here give me some suggestion as whether this would affect the outcome of the experiment? What would be a decent size difference to expect when doing multiplexing? I also found that the input samples tend to have more such variations.
Many thanks.
Ping
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