Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Hello from NuGEN Technologies

    Steve Kain here. We have recently introduced a solution for RNA-Seq sample prep with inputs of total RNA in the range of 500 pg to 100 ng. The product is double-stranded cDNA in the size range of 50-1,500 bases. I look forward to monitoring this site to learn more about NGS sample prep needs, and progress in the field in general. We are located in the SF Bay Area.

    Cheers,
    Steve

  • #2
    Compatibility with SOLiD?

    Hi Steve,
    Is the Ovation RNA-Seq kit compatible with the SOLiD sequencers? We are working with pg quantities of RNA at the moment and will be lucky to get 1ng at the end and want to screen for miRNAs.
    Cheers

    Comment


    • #3
      Yes, compatible with SOLiD and 454

      The product from the Ovation RNA-Seq workflow is ds cDNA, and hence compatible with any library prep system using this starting material. We have several customers that have used the system successfully on SOLiD.

      Best,
      Steve

      Comment


      • #4
        specific question

        Steve,

        Could Prelude be used with OCT-embedded tissue samples (very tiny tissue samples)? For OCT-embedded frozen samples, would one use:
        Ovation® RNA-Seq FFPE System
        Ovation® RNA-Seq
        Ovation® 3'-DGE System

        We want have 24 paired samples (12 pairs), and would like to get gene expression data from these samples. Using Qiagen's micro kit, we have RINs and yields ranging from 2.3-5.9 RIN, 9.1 to 2140 mass (ng).

        Thanks,
        Anand

        Comment


        • #5
          Hi Anand,

          Can you please provide some details regarding the impact of OCT treatment on the samples? Ovation RNA-Seq FFPE is preferable if you have degraded samples, and/or cross-linking typically brought about by formalin fixation.

          Best,
          Steve

          Comment


          • #6
            Hi,

            What is different from the 'normal' rna-seq kit to the FFPE ?

            Thanks

            Comment


            • #7
              I forgot, is there anyone in here that used the FFPE kit with succes?

              thanks

              Comment

              Latest Articles

              Collapse

              • seqadmin
                Strategies for Sequencing Challenging Samples
                by seqadmin


                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                03-22-2024, 06:39 AM
              • seqadmin
                Techniques and Challenges in Conservation Genomics
                by seqadmin



                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                Avian Conservation
                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                03-08-2024, 10:41 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by seqadmin, Yesterday, 06:37 PM
              0 responses
              10 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, Yesterday, 06:07 PM
              0 responses
              10 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-22-2024, 10:03 AM
              0 responses
              51 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-21-2024, 07:32 AM
              0 responses
              67 views
              0 likes
              Last Post seqadmin  
              Working...
              X