Hi,
I'm working with 5bp + T Tags for multiplexing 2-4 samples in each line.
In my first run I don't find the exact sequence of the Tags. Actually I find the Tags without the first base. I don´t understand this circumstance because the sequence of PCR and sequencing primers end one base before the Tags.
What can be happening?
Thanks!
I'm working with 5bp + T Tags for multiplexing 2-4 samples in each line.
In my first run I don't find the exact sequence of the Tags. Actually I find the Tags without the first base. I don´t understand this circumstance because the sequence of PCR and sequencing primers end one base before the Tags.
What can be happening?
Thanks!
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