SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Problem with uneven distribution of rads among individual libraries apfuentes Sample Prep / Library Generation 10 03-22-2016 08:18 AM
Miseq v2.2 300 cycle cartridges back-ordered? DHFK Illumina/Solexa 6 08-12-2015 07:55 AM
MiSeq v2 cartridges for metagenomics Allison Harmon Metagenomics 4 11-23-2014 08:30 AM
help! RAD, Stacks process_radtags.pl script, ambiguous RADs nsaarman Bioinformatics 0 04-05-2013 12:15 PM
multiple cartridges in a single MiSeq run? wingtec General 1 08-10-2012 01:21 PM

Reply
 
Thread Tools
Old 03-26-2018, 11:51 AM   #1
minio.cz
Junior Member
 
Location: Prague

Join Date: Jul 2015
Posts: 5
Default Combining MiSeq cartridges to get longer rads

Hi all,
I would like to combine three 50 reads cartridges (we have half of freezer of expired ones) to get 2x75bp reads. I know it is possible and that it will work and used to have publication where they were making 1200bp reads on MiSeq. But I cannot find it now, so I would like to ask what wells I have to combine and if I have to disassemble the cartridge (not easy) or if I can just puncture the tinfoil and combine them using pasteur pipette.
minio.cz is offline   Reply With Quote
Old 04-04-2018, 03:06 AM   #2
Markiyan
Senior Member
 
Location: Cambridge

Join Date: Sep 2010
Posts: 115
Default First get an RFID override code...

1. you would need an RFID override code from the Illumina support.
2. The pasteur pipette should work ok (make sure it is sterile and protease/DNA'se free!)
3. If trying longer reads (>250bp), try lowering the clustering density to get a better quality and lower dephasing...
4. Keep the room with the miseq on a cooler side - 18 degrees or so.
Markiyan is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:28 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO