Hi, I have been using several scripts from maq, fastq-mcf and galaxy to transform my csfasta & qual data into fastq (in base space). all of them gave me the same sequence, but when I tried to do it manually based on the code given by Solid I get a different sequence. I tried a script that I found in a forum and is the only script that has coincide with my translation. Can anyone tell me which is the right conversion and which one has a frame shift please?
original .csfasta
@373_15_137_F3
T32330320332012322113122303202132003331331003110331
manual
@373_15_137_F3
AGCGGCTTATCCAGCTCACGTCTAATCCTGCTTTATACGCAAATGTTATG
scripts: maq,galaxy, fastq-mcf
@373_15_137_F3
TTGTTATGATTGACGTGGCCTCGGTATGAGCTGAATTTCTTCAATCCATTC
original .csfasta
@373_15_137_F3
T32330320332012322113122303202132003331331003110331
manual
@373_15_137_F3
AGCGGCTTATCCAGCTCACGTCTAATCCTGCTTTATACGCAAATGTTATG
scripts: maq,galaxy, fastq-mcf
@373_15_137_F3
TTGTTATGATTGACGTGGCCTCGGTATGAGCTGAATTTCTTCAATCCATTC
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