Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • strange bowtie index building and mapping problem

    Hello everyone,
    I have met a quite strange problem when running bowtie for my SOLID sequencing result.
    I have succeeded to build bowtie colorspace index for chimpanzee whole genome, but when I run bowtie , it showed the error like:
    "run_bowtie.sh: line 32: 1898 Segmentation fault $bowtie -C --phred33-quals -n 3 -a -m 3 --best --strata -p 4 -S $index -q $infile $out_dir/$out_name
    "
    Then I checked the output, the output just contain sam header and a few line of alignment result. The strange thing is that the sam header is like this(chromosome name is incorrect):
    @HD VN:1.0 SO:unsorted
    @SQ SN:IB<8D><EA>^RP<F3>{<<FD><FA><93>ދ<FC>^X%^M<C3>^R^V<DA>A LN:135001995
    @SQ SN:1 LN:8402541
    @SQ SN:2 LN:134204764
    @SQ SN:3 LN:8412303
    @SQ SN:4 LN:135371336
    @SQ SN:5 LN:2259969
    @SQ SN:6 LN:115868456
    @SQ SN:7 LN:9231045
    @SQ SN:8 LN:107349158
    @SQ SN:9 LN:2108736
    @SQ SN:10 LN:100063422
    @SQ SN:11 LN:3087076
    @SQ SN:12 LN:90682376
    @SQ SN:13 LN:6370548
    @SQ SN:14 LN:83384210
    @SQ SN:15 LN:5078517
    @SQ SN:16 LN:77261746
    @SQ SN:17 LN:1841920
    @SQ SN:18 LN:64473437
    @SQ SN:19 LN:2407237
    ......

    I have done same procedure for human, the sam header is :
    @HD VN:1.0 SO:unsorted
    @SQ SN:chr10 LN:135534747
    @SQ SN:chr11 LN:135006516
    @SQ SN:chr11_gl000202_random LN:40103
    @SQ SN:chr12 LN:133851895
    @SQ SN:chr13 LN:115169878
    @SQ SN:chr14 LN:107349540
    @SQ SN:chr15 LN:102531392
    @SQ SN:chr16 LN:90354753
    @SQ SN:chr17_ctg5_hap1 LN:1680828
    .....

    So it seems bowtie either didn't build a correct colorspace index or read the index incorrectly. By the way, I have checked the chimpanzee genome I used, it seems OK(the chromosome name is chr1,chr2a, .....chrX, ...).

    Have anybody met the same problem before? I am quite precious to hear your suggestion.

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-06-2024, 07:17 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
22 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
24 views
0 likes
Last Post seqadmin  
Working...
X