Hi All,
I'm sequencing ~36 metagenomes and I'm thinking about how to get the best coverage.
The easiest thing would be to pool all 36 and run them across lanes - this will also help with lane to lane variation. But I think this will reduce the overall sequencing depth of each sample (index). As I'll just be resequencing the more abundant sequences (organisms) over and over.
So will it actually help to run say 8 samples per lane to make sure that the same clusters are not being sequenced again and again.
Thanks
I'm sequencing ~36 metagenomes and I'm thinking about how to get the best coverage.
The easiest thing would be to pool all 36 and run them across lanes - this will also help with lane to lane variation. But I think this will reduce the overall sequencing depth of each sample (index). As I'll just be resequencing the more abundant sequences (organisms) over and over.
So will it actually help to run say 8 samples per lane to make sure that the same clusters are not being sequenced again and again.
Thanks
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